BANBURY United began the season with a 3-0 defeat on home soil.
Visiting side Spennymoor Town scored all their goals in a 13-minute spell during the second half.
The Moors had the ball in the back of the net after nine minutes, but it was ruled out for offside.
Glen Taylor put the away team in front with 55 minutes on the clock though, before Danny Greenfield made it 2-0 when he finished a counter-attack which started from a Banbury corner.
Moments later, Taylor completed his brace as slotted home a penalty awarded after a foul by Yonis Farah.
READ ALSO: Oxford City beaten in first ever National League game
After the game, Puritans boss Mark Jones told the club social media channels: “Obviously we’re disappointed, we don’t like losing and its’ not the way we wanted to start the season.
“We have to take it on the chin and we have to look to improve.
“I thought we were well in the game and it was a tight game in the first half.
“We had some good moments and they did too.
“We didn’t start the second half particularly well and they pinned us in.
“We conceded that first goal fairly early in the second half and what we needed to do then was stay in the game but unfortunately we conceded the second goal pretty quickly after the first, and that took the game from us.
“We must learn from it and bounce back stronger.”
Didcot Town marked their return to Southern League Premier South with a narrow home defeat.
Sam Ashton scored the only goal of the game at the Loop Meadow Stadium, as visitors Hayes & Yeading United left with all three points.
Shortly after the hour mark, Tarik Moore-Azille was shown a second yellow card for the away team, who managed to hold on.
In the Emirates FA Cup extra preliminary round, Kidlington were beaten 3-0 at home to Flackwell Heath.
Fabian Drifill gave the away team the lead just before half-time, with Marcus Mealing doubling the lead from a free kick.
Ben Richards then picked up his second yellow card for Kidlington, before Mealing made it three from the penalty spot.
Thame United lost 3-2 at home against Bristol Manor Farm.
Both Oxfordshire sides will receive £375 for bowing out at this stage of the competition.
Help support trusted local news
Sign up for a digital subscription now: oxfordmail.co.uk/subscribe
As a digital subscriber you will get:
- Unlimited access to the Oxford Mail website
- Advert-light access
- Reader rewards
- Full access to our app
Comments: Our rules
We want our comments to be a lively and valuable part of our community - a place where readers can debate and engage with the most important local issues. The ability to comment on our stories is a privilege, not a right, however, and that privilege may be withdrawn if it is abused or misused.
Please report any comments that break our rules.
Read the rules here