Junior doctors in Buckinghamshire, Oxfordshire and Berkshire West will start a five-day strike later this month.
The industrial action will take place from 7am on Saturday, February 24, until 11.59pm on, Wednesday, February 28.
The strike will involve members of the British Medical Association union and could cause significant disruption to health services.
The strike will likely impact routine and planned services, despite work across the NHS to protect essential areas such as emergency, critical care, maternity and trauma.
Meanwhile, members of the Hospital Consultants and Specialists Association union are due to end their strike at 7am on Thursday, February 29.
Dr Nick Broughton, interim chief executive of the NHS Buckinghamshire, Oxfordshire and Berkshire West Integrated Care Board, said: "The walkout will sadly once more bring significant challenges to our services.
"Emergencies, critical care, maternity, trauma and neonatal care safe staffing will be our absolute priority."
Patients with scheduled appointments are advised to attend unless notified otherwise.
To reduce the strain on services, the NHS encourages the public to use the 111 online service or call 111.
Dr Rachael de Caux, chief medical officer at the NHS Buckinghamshire, Oxfordshire and Berkshire West Integrated Care Board, said: "Patients who need urgent medical care must continue to come forward as normal, especially in emergency and life-threatening cases.
"It may take longer during this period to be seen and treated so we ask you to please be patient."
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